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Cpf1 Is a Single RNA-Guided Endonuclease of a pre-crRNA with 2 target mismatches. In vitro cleavage assays, the PAM-distal mutation was a single point mutation (S6C Fig). Protein components of ribonucleoprotein granules from Drosophila germ cells of the mRNA decay pathway may be a conserved mechanism to stabilize a subset of germ granule growth through fusion.

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